Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

EEF2K (O00418) - Overview - Molecular Target Synopsis

Protein


EEF2K, Eukaryotic elongation factor 2 kinase
Enzyme Classification 2.7.11.20
UniProt O00418

Also Known as EF2K_HUMAN, EEF2K

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. Monomer or homodimer (Probable). Interacts with Calmodulin/CALM1; this interaction is strictly required for phosphorylation activity (PubMed:11015200).

6NX4
STRUCTURE OF THE C-TERMINAL HELICAL REPEAT DOMAIN OF EUKARYOTIC ELONGATION FACTOR 2 KINASE (EEF-2K)
RCSB/PDB
Inspect Structure
See all 3D Structures for EEF2K

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
725ENSG00000103319ENST00000263026ENSP00000263026O00418-1
412ENSG00000103319ENST00000568269ENSP00000456243

Sub-cellular localization


GO terms: EEF2K is active in the following subcellular-locations: cytoplasm, cytosol, dendritic spine, postsynaptic density.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project EEF2K has gain in 1 cell-lines, loss in 1 cell-lines and no signal in 1003 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: RPMI_8226, M14, OVCAR_4

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: NCI-H82, G122, MS751

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, NHLF, K562

(see details)

RNA Interference


EEF2K was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: HELA, CASKI. (see details)

3D Structures


For EEF2K there are:
3 structures (3 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


EEF2K has been screened with 400 compounds (624 bioactivities), 11 compounds have bioactivities that show binding affinity of <= 500nM (12 bioactivities). (see details)