Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

CNOT1 (A5YKK6) - Overview - Molecular Target Synopsis

Protein


CNOT1, CCR4-NOT transcription complex subunit 1
UniProt A5YKK6

Also Known as CNOT1_HUMAN, CNOT1, CDC39, KIAA1007, NOT1

Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity. Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits (PubMed:10637334, PubMed:16778766, PubMed:19558367, PubMed:22977175). In the complex, interacts directly with CNOT6, CNOT6L, CNOT7 or CNOT8 (PubMed:10637334, PubMed:16778766, PubMed:22977175). Interacts in a ligand-dependent fashion with ESR1 and RXRA (PubMed:16778766). Interacts with NANOS2, TOB1 and ZFP36 (PubMed:18377426, PubMed:21278420, PubMed:23644599). Interacts with TNRC6A, TNRC6B or TNRC6C; the interactions are direct (PubMed:21981923, PubMed:21984185). Interacts with YTHDF2; the interaction is direct and promotes recruitment of the CCR4-NOT complex to N6-methyladenosine (m6A)-containing mRNAs, leading to their deadenylation and subsequent degradation (PubMed:27558897).

5ONA
DROSOPHILA BAG-OF-MARBLES CBM PEPTIDE BOUND TO HUMAN CAF40-CNOT1
RCSB/PDB
Inspect Structure
See all 3D Structures for CNOT1

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: CNOT1 is active in the following subcellular-locations: cytoplasm, nucleus, p-body.
GO terms: CNOT1 is active in the following subcellular-locations: CCR4-NOT complex, CCR4-NOT core complex, cytosol, extracellular space, membrane, nucleus, P-body, peroxisomal membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project CNOT1 has gain in 1 cell-lines, loss in 1 cell-lines and no signal in 1003 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: SW_620, HL_60, OVCAR_8

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: HCC78, SiHa, KYSE-70

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: HeLa-S3, NHLF, HSMM

(see details)

3D Structures


For CNOT1 there are:
15 structures (27 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


CNOT1 has been screened with 3 compounds (4 bioactivities). (see details)